MacConkey's Agar Growing E. coli, Enterobacter & Salmonella (clockwise from top left plate)
MacConkey's Agar Growing Gram-negative Bacteria: Lactose Fermenter on Right, Non-lactose Fermenter on Left.

MacConkey's Agar (MAC) 
Differential & Selective Bacterial Growth Medium

LAB NOTES from Science Prof Online


When bacterial cultures are grown in a laboratory, they are growing in a captive environment, like a tiny microbial zoo. These captive-bred bacteria are totally dependent on people to provide the proper environment for their survival and growth. A nutrient-rich media is required to grow bacteria in the lab.

Article Summary: MacConkey's Agar is a specialized bacterial growth medium that is selective for Gram-negative bacteria and can differentiate those Gram- bacteria that are able to ferment lactose.
How to Interpret MacConkey's Agar (MAC)
MacConkey's Agar Growing E. coli, Enterobacter & Salmonella (clockwise from top left plate).
MacConkey's Agar (MAC): 
Growing E. coli (bright pink), 
Enterobacter (pink margins) 
Salmonella (colorless), 
clockwise from top left plate.
Click here for more 
MAC images.
Page last updated: 5/2015
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 1. Sterile plate of MacConkey's agar; 2. Kitchen sink sample plated in TSY (front) and MAC (back, note very pink Gram-negative lactose fermenters); 3. E. coli, happy to be plated on MAC; 4. Salmonella (G -, lac -) plated on MAC; 5. E. coli (G -, lac +) plated on MAC. Click here for more photos.
Differential and selective media are special types of agar that can can exclude certain types of bacteria and even test for certain bacterial metabolic capabilities. MacConkey’s (MAC), Blood agar  (BAP) and Mannitol Salt (MSA) are three examples of these specialized types of media.
VIDEO: ​How to Interpret MacConkey's Agar (MAC) Bacterial Growth Medium
Instructor's Corner
What is Bacterial Growth Media?
growth medium (plural: media) is a mixture of nutrients, moisture and other chemicals that bacteria need for growth. Media are used to grow bacterial colonies (millions of bacteria having arisen through the binary fission of a single progenitor).

​Using Media to Identify Bacteria
Like the differential staining of bacteria, special types of media can be used to provide clues about a microbe’s identity. There are many types of media that are specific about what they grow, or that provide information about the type of microbes present.

Microbiology Lab Videos
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See Page 2 for FREE homework assignment and other free teaching materials on Specialized Bacterial Growth Media!
Bacteria, known as “lactose fermenters”, eat the media’s lactose, and, in the process, create an acidic end product that causes the pH indicator, neutral red, to turn pink. With MacConkey’s, it is not the media that changes color, but rather the actual colonies of lactose fermenting bacteria that appear pink. Non-lactose fermenting bacteria will be colorless (or, if they have any color, will be their natural color rather than pink).
Selective and Differential Media
If a bacterial growth medium is selective, that means that it grows only certain types of microbes while inhibiting the growth of others. Agar is considered a differential growth medium if, when specific microbes are present, the medium or bacterial colonies themselves exhibit a color change that provides information about their identity.

MacConkey's Agar Is Selective
MacConkey's is a selective medium that inhibits the growth of Gram-positive bacteria due to the presence of crystal violet and bile salts. Gram-negative bacteria grow well on MAC.

MacConkey's Agar Is Differential
MAC is also a differential, meaning that it differentiates or distinguishes between groups of bacteria on the basis of a color change reaction. MacConkey’s contains two additives that make it differential; neutral red (a pH indicator) and lactose (a disaccharide).

Sterile Specialized Bacterial Growth Media. Clockwise from top left MacConkey's, Mannitol Salt and Blood Agar
Sterile Specialized Bacterial Growth Media. Clockwise from top left MacConkey's, 
Mannitol Salt and Blood Agar.
More Photos of MacConkey's Bacterial Growth Medium