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Gram Staining & Isolation Streak Plate Method
Identification of Unknown Bacteria Laboratory Exercise
Lab #2: Gram Stain & Isolation Streak Plate
This is the main page for the Gram Stain & Isolation Streak Plate Laboratory Topic of an 8-week introductory college microbiology class. The links on this page provide the Virtual Microbiology Classroom documents, web pages and videos useful in preparing for this lab.
You have free access to a large collection of materials used in a college-level introductory microbiology course. The Virtual Microbiology Classroom provides a wide range of free educational resources including PowerPoint Lectures, Study Guides, Review Questions and Practice Test Questions.
SCIENCE PHOTOS
SPO VIRTUAL CLASSROOMS
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ADDITIONAL LABORATORY REQUIRED READINGS
Microscopy:
Bacterial Stains:
- Gram Stain Procedure: Differential Stain for Gram+ & Gram- Bacteria Identification
- Differential Staining & Bacterial Controls: Gram, Acid-fast & Endospore Stains
Bacterial Media & Culture:
How to Prepare a Bacterial Smear for Gram Staining
How to Do a Gram Stain
Compound Light Microscope Parts & Operation Tutortial
MORE GRAM STAIN & ISOLATION STREAK PLATE LINKS
- Streak Plate Procedure Animation: with narration from Sinauer Associates.
- Streak Plate Interactive Animation from MSU. Test your skill to see if you can do a virtual streak plate procedure that produces isolated colonies.
- Gram Stain Bite Sized Tutorial: This is an extremely useful tutorial that shows, step-by-step, the Gram-staining procedure and the appearance of Gram+ and Gram- bacterial cells.
GRAM STAIN LABORATORY VIDEOS
GRAM STAIN LABORATORY IMAGES
The images linked here directly relate to the lab exercise and are designed to help you review and study that lab material while outside of lab.
SCIENCE VIDEOS
NOTE: Students must review all material in categories highlighted with red titles PRIOR TO ATTENDING LAB in order to be prepared to participate.
GRAM STAIN & ISOLATION STREAK PLATE
LABORATORY DOCUMENTS
The Lab Exercise Instructions are the main resource required for students to understand and complete this exercise. They provide a thorough explanation of the lab topic, as well as detailed directions on how to complete the exercise. The Lab Report Assignment is the homework that students complete and turn in.
- Lab #2: Gram Stain & Isolation Streak Plate Lab Exercise INSTRUCTIONS
- Lab #2: Gram Stain & Isolation Streak Plate Lab Report ASSIGNMENT
Additional Lab Documents:
GRAM STAIN & ISOLATION STREAK PLATE LAB POWERPOINTS
The lab PowerPoints used to introduce the material in the related Lab Exercise Instructions & Report Assignment.
- Lab #2: Gram Staining Procedure [PowerPoint Show or PDF Printout]
- Lab #2: How to Prepare an Isolation Streak Plate [PowerPoint Show or PDF Printout]
Printing: The best way for students to print out the PowerPoint Show is to download the PDF version. Select Print, and, when the Print screen comes up, go to the Print Handling options. Under Page Scaling, select "Multiple pages per sheet", Under Pages per sheet, select "2". That uses up less paper, placing two slides on each page.
The Gram Stain & Isolation Streak Plate Lab materials include:
- Lab Exercise & Report word documents
- Laboratory PowerPoints
- Additional laboratory required readings
- Videos and images related to the lab
The purpose of isolation streak plate technique is to spread out bacteria so that individual colonies can be seen.
Page last updated:
2/2016
Gram negative
E. coli @ 1000xTM
Gram positive
Staph @ 1000xTM
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