Microbiology Class Laboratory Materials

Virtual Microbiology Classroom from Science Prof Online

MacConkey's Agar with Lac- Salmonella on top and Lac+ Ecoli on bottom
MacConkey's Agar with Lac- Salmonella on top and Lac+ Ecoli on bottomMacConkey's Agar with Lac- Salmonella on top and Lac+ Ecoli on bottom
MacConkey's Agar with Lac- Salmonella on top and Lac+ Ecoli on bottomMacConkey's Agar with Lac- Salmonella on top and Lac+ Ecoli on bottomMacConkey's Agar with Lac- Salmonella on top and Lac+ Ecoli on bottom
MacConkey's Agar with Lac- Salmonella on top and Lac+ Ecoli on bottomMacConkey's Agar with Lac- Salmonella on top and Lac+ Ecoli on bottomMacConkey's Agar with Lac- Salmonella on top and Lac+ Ecoli on bottomMacConkey's Agar with Lac- Salmonella on top and Lac+ Ecoli on bottom
MacConkey's Agar with Lac- Salmonella on top and Lac+ Ecoli on bottomMacConkey's Agar with Lac- Salmonella on top and Lac+ Ecoli on bottomMacConkey's Agar with Lac- Salmonella on top and Lac+ Ecoli on bottom
MacConkey's Agar with Lac- Salmonella on top and Lac+ Ecoli on bottomMacConkey's Agar with Lac- Salmonella on top and Lac+ Ecoli on bottom
MacConkey's Agar with Lac- Salmonella on top and Lac+ Ecoli on bottom
MacConkey's Agar with Lac- Salmonella on top and Lac+ Ecoli on bottomMacConkey's Agar with Lac- Salmonella on top and Lac+ Ecoli on bottom
Mannitol Salt Bacterial Growth Media:
Only grows halophilic (salt-loving) bacteria. Plate on left has turned yellow, because the bacteria growing is Staph aureus, a mannitol fermenter.Plate on right also has growth, but remains pink, indicating Staph epidermidis, a non-mannitol fermenter. 
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More Useful Microbiology Resources







Page last updated 2/2015



Photographic guides to differential stains 
now available!
1. Gram Stain
2. Acid-fast Stain
3. Endopsore Stain

What might happen if a deadly new infectious disease hit the scene? 

The film "Contagion" provides an excellent opportunity to learn about infectious disease, how it spreads and which organizations would be involved in tracking and controlling a dangerous outbreak.

Completing the Contagion Extra Credit Assignment will help you better understand this complex film.
Go to Virtual Microbiology Classroom
SPO VIRTUAL CLASSROOMS
This page features the laboratory materials from Virtual Microbiology Classroom

There are also free 
lecture teaching materials and 
microbiology reading recommendations 
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Microbiology Lab Videos
SCIENCE VIDEOS
Virtual Microbiology Classroom LABORATORY TOPICS

LAB Exercise: How to Use a Compound Microscope
Basic microscopy and viewing bacterial simple stain.

FREE Microbiology Laboratory Exercises

Science Prof Online offers FREE fully-developed science curricula, including the Virtual Microbiology Classroom (8-week and 16-week micro courses), the Virtual Cell Biology Classroom and the Virtual Anatomy & Physiology Classroom (coming soon).

LAB Exercise: Photomicroscopy, Leukocytes & the Wright Stain
Students review basic microscopy, learn how to obtain photomicrographs and examine leukocytes in a Wright stain.

LAB Exercise: Gram Staining & Isolation Streak Plate Method
First lab in the series on identifying unknown bacteria. Students learn how to create a bacterial smear and preform the Gram stain, with controls and an unknown. Isolation streak plate technique also introduced.

LAB Exercise: Differential Staining & Specialized Media
Second lab in a series on identifying unknown bacteria. Students are introduced to the Acid-fast and Endospore stains. Specialized media (MacConkey's & Mannitol Salt) are inoculated with unknown to assist in identification of unknown bacteria using a dichotomous key.

LAB Exercise: Bacterial Growth Media & Cultures
Third lab in a series on identifying unknown bacteria. Students learn how to create controls showing various bacteria plated on MacConkeys, Mannitol Salt, Blood Agar & Tryptic Soy Agar. Students collect and interpret samples of normal flora from their own bodies.
LAB Exercise: Urine Cultures & Bacterial Identification
Students test mock urine cultures of stock microbes with identities unknown to them. They choose which tests are required to identify their microbe. Students exposed to Gram staining, antibiotic sensitivity testing, api-20e strip and use of a calibrated loop and spreader.

LAB Exercise: Testing Amount of Bacteria on Chicken Wings
Students obtain washes of chicken wings and perform dilutions to estimate actual amount of bacteria present.
LAB Exercise: Microbial Control: How to Kill Bacteria
Experiments in using heat, ultra-violet radiation, antibiotics and chemical disinfectants to kill bacteria.

LAB Exercise: Immunology - Leukocytes, Antibodies & Blood Typing
​Students learn about the cells involved in immune response, as well as antibody-antigen interaction by playing a virtual blood typing game and viewing leukocytes with the microscope.

Additional Microbiology Laboratory Resources

Microbiology Reading 
Recommendations








Spillover by David Quammen
Rabid by Wasik & Murphy
The Viral Storm by Wolfe
The Ghost Map by Johnson
​- Missing Microbes by Blaser
  - The Great Mortality by John Kelly
-The Great Influenza by Berry
Pandemics: What Everyone Needs to Know by Doherty
Deadly Outbreaks by Levitt
Microbe Hunters by De Kruif
Wickett's Remedy by Goldberg
The Dress Lodger by Holman

Spillover Quammen
Virtual Microbiology
Classroom

8 week or 16 week course





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The Virtual Microbiology Classrooms provide a wide range of FREE educational resources including PowerPoint Lectures, Study Guides,  Practice Test Questions, 
Microbiology Images & Videos.
Ermahgerd Microbiology Science
SPO VIDEO: 
How to Do a Gram Stain
SPO VIDEO: Tutorial on Compound Light Microscope Parts & Operation
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