Science Image Library: Gram Stain

Science Image Library
Gram Stain

from Science Prof Online
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Gram-stained Staphylococcus @1000xTM

1. Crystal violet, the primary stain in Gram-staining; 2. Rinsing between steps of the Gram stain; 3. Application of iodine mordant during Gram stain; 4 & 5. Application of Gram counterstain, safanin.
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Prokaryotic Cell, Mariana Ruiz
Gram Stain Procedure (Click on image to enlarge.)
Gram Stained Slides (Click on image to enlarge.)
1 & 2. Gram stained slides, showing + & - bacterial controls on left and right circles of slide and unknown bacteria in center.
Gram-positive Bacteria Under Microscope (Click on image to enlarge.)
1 & 2. Gram stain of StaphylococcusGram-positive @ 1000xTM; 3. Gram stain of Staphylococcus, Gram+ @ 1000xTM+; Gram+ specimen viewed at 100xTM; Gram+ specimen viewed at 400xTM.
Gram-negative Bacteria Under Microscope (Click on image to enlarge.)
1 & 2. Gram stain of E. coli, Gram- @ 1000xTM; 3. Gram stain of Gram-negative bacterial sample @ 1000xTM+.
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Mixed Gram + & Gram- Bacteria Under Microscope (Click on image to enlarge.)
1 & 2. Gram stain mixed sample od Staphylococcus (Gram +, purple cocci) & E. coli, (Gram- pink rods) @ 1000xTM
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How to Do a Gram Stain
VIDEO: How to Prepare a Bacterial Smear for Gram Staining
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Page last updated 5/2014
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Gram Positive Bacteria, Staph. epidermidis @1000xTM
Gram-positive Staphylococcus epidermidis @ 1000xTM+
Gram Negative Bacteria, E. coli @ 1000xTM
Gram-negative Escherichia coli @ 1000xTM
Additional Gram Staining Resources
  • Gram Stain Bite Sized Tutorial: This is an extremely useful tutorial that shows, step-by-step, the Gram-staining procedure and the appearance of Gram+ and Gram- bacterial cells.



Photographic guides to differential stains 
now available!
1. Gram
2. Acid-fast
3. Endopsore